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<t>Holographic</t> optical tweezers (A) Schematics of the HOTs coupled to an inverted microscope. SLM is the spatial light modulator, M i mirrors, L i lenses, λ / 2 half-wave plate, Pol linear polarizer, D dichroic mirror, Ob1 condenser objective, Ob2 imaging objective, that also focuses the laser beam on the sample plane ( x , y ) . (B) Schematics of single erythrocyte trapped by a laser hologram that reproduces the membrane contour. E ( x , y ) is the trapping potential. Red arrows indicate the laser propagation, yellow arrows represent the direction of in plane optical forces. (C) Image of an RBC in the equatorial plane without laser trapping. The membrane contour is retrieved as the high-contrast border between inner (black) and outer (white) circles (see ). Scale bar is 2 μ m .
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<t>Holographic</t> optical tweezers (A) Schematics of the HOTs coupled to an inverted microscope. SLM is the spatial light modulator, M i mirrors, L i lenses, λ / 2 half-wave plate, Pol linear polarizer, D dichroic mirror, Ob1 condenser objective, Ob2 imaging objective, that also focuses the laser beam on the sample plane ( x , y ) . (B) Schematics of single erythrocyte trapped by a laser hologram that reproduces the membrane contour. E ( x , y ) is the trapping potential. Red arrows indicate the laser propagation, yellow arrows represent the direction of in plane optical forces. (C) Image of an RBC in the equatorial plane without laser trapping. The membrane contour is retrieved as the high-contrast border between inner (black) and outer (white) circles (see ). Scale bar is 2 μ m .
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in-house physical optics (po) code  (MathWorks Inc)
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<t>Holographic</t> optical tweezers (A) Schematics of the HOTs coupled to an inverted microscope. SLM is the spatial light modulator, M i mirrors, L i lenses, λ / 2 half-wave plate, Pol linear polarizer, D dichroic mirror, Ob1 condenser objective, Ob2 imaging objective, that also focuses the laser beam on the sample plane ( x , y ) . (B) Schematics of single erythrocyte trapped by a laser hologram that reproduces the membrane contour. E ( x , y ) is the trapping potential. Red arrows indicate the laser propagation, yellow arrows represent the direction of in plane optical forces. (C) Image of an RBC in the equatorial plane without laser trapping. The membrane contour is retrieved as the high-contrast border between inner (black) and outer (white) circles (see ). Scale bar is 2 μ m .
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<t>Holographic</t> optical tweezers (A) Schematics of the HOTs coupled to an inverted microscope. SLM is the spatial light modulator, M i mirrors, L i lenses, λ / 2 half-wave plate, Pol linear polarizer, D dichroic mirror, Ob1 condenser objective, Ob2 imaging objective, that also focuses the laser beam on the sample plane ( x , y ) . (B) Schematics of single erythrocyte trapped by a laser hologram that reproduces the membrane contour. E ( x , y ) is the trapping potential. Red arrows indicate the laser propagation, yellow arrows represent the direction of in plane optical forces. (C) Image of an RBC in the equatorial plane without laser trapping. The membrane contour is retrieved as the high-contrast border between inner (black) and outer (white) circles (see ). Scale bar is 2 μ m .
Spectrophotometer Konica Minolta Cm 3700a, supplied by Konica Minolta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Holographic optical tweezers (A) Schematics of the HOTs coupled to an inverted microscope. SLM is the spatial light modulator, M i mirrors, L i lenses, λ / 2 half-wave plate, Pol linear polarizer, D dichroic mirror, Ob1 condenser objective, Ob2 imaging objective, that also focuses the laser beam on the sample plane ( x , y ) . (B) Schematics of single erythrocyte trapped by a laser hologram that reproduces the membrane contour. E ( x , y ) is the trapping potential. Red arrows indicate the laser propagation, yellow arrows represent the direction of in plane optical forces. (C) Image of an RBC in the equatorial plane without laser trapping. The membrane contour is retrieved as the high-contrast border between inner (black) and outer (white) circles (see ). Scale bar is 2 μ m .

Journal: iScience

Article Title: Red blood cell flickering activity locally controlled by holographic optical tweezers

doi: 10.1016/j.isci.2024.109915

Figure Lengend Snippet: Holographic optical tweezers (A) Schematics of the HOTs coupled to an inverted microscope. SLM is the spatial light modulator, M i mirrors, L i lenses, λ / 2 half-wave plate, Pol linear polarizer, D dichroic mirror, Ob1 condenser objective, Ob2 imaging objective, that also focuses the laser beam on the sample plane ( x , y ) . (B) Schematics of single erythrocyte trapped by a laser hologram that reproduces the membrane contour. E ( x , y ) is the trapping potential. Red arrows indicate the laser propagation, yellow arrows represent the direction of in plane optical forces. (C) Image of an RBC in the equatorial plane without laser trapping. The membrane contour is retrieved as the high-contrast border between inner (black) and outer (white) circles (see ). Scale bar is 2 μ m .

Article Snippet: Code for driving holographic optical tweezers (MATLAB) , https://figshare.com/ , https://doi.org/10.6084/m9.figshare.24973908.

Techniques: Inverted Microscopy, Imaging, Membrane

Journal: iScience

Article Title: Red blood cell flickering activity locally controlled by holographic optical tweezers

doi: 10.1016/j.isci.2024.109915

Figure Lengend Snippet:

Article Snippet: Code for driving holographic optical tweezers (MATLAB) , https://figshare.com/ , https://doi.org/10.6084/m9.figshare.24973908.

Techniques: Software, Membrane